P-667 cpDNA spectinomycin resistant spr-u-1-6-2

$30.00

Boynton-Gillham laboratory, Duke University, 1997

Chloroplast DNA Bam-HindIII (4.8 kb) in pUC18, 16S rRNA through 5′ part of 23S rRNA; contains spectinomycin resistance mutation spr-u-1-6-2, from CC-110, in 3′ end of 16S rRNA; same insert as P-228 but in XL1 blue host strain

host strain: XL1-Blue
amp resistant

 

More information

Strain: CC-110 spr-u-1-6-2 mt+

Insert: BamHI-HindIII (4.8 kb) from BamHI 11/12

Genes present: 16S rRNA, 23Ss rRNA (5′ end)

Vector: pUC18

Bacterial host strain: XL1 blue

Selectable marker: amp-r, white on X-gal

Origin: Scott Newman in Boynton/Gillham lab, 1989; transferred to XL1 blue by Barbara Randolph-Anderson, 1995
P-667

This is the same insert as P-228, but in XL1 blue

The spr-u-1-6-2 mutations is an A-> G change at bp 1123 in the C. reinhardtii 16S rRNA gene which causes loss of an AatII restriction site and confers high level spectinomycin resistance. See Harris et al., Genetics 123, 281-292 (1989). Used in rRNA transformation experiments by Newman et al., Genetics 126, 875-888 (1990).