* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-739 cpDNA atpB, with atpE 5′ (no S-D) : aadA : rbcL 3′
$30.00
Boynton-Gillham laboratory, Duke University, 1998
E. coli aadA gene flanked by C. reinhardtii atpE 5′ (with no Shine-Dalgarno sequence) and rbcL 3′; inserted downstream of the atpB gene but in the opposite orientation; used to transform an atpB deletion mutant. Eco-Xho in pUC18.
host strain: XL1-Blue
amp resistant
Fargo DC, Zhang M, Gillham NW, Boynton JE (1998) Shine-Dalgarno-like sequences are not required for translation of chloroplast mRNAs in Chlamydomonas reinhardtii chloroplasts or in Escherichia coli. Mol Gen Genet 257:271-282