* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pAES5
$30.00
From Anthony Berndt, Stephen Mayfield Lab, University of California, San Diego, November 2021
Vector: pUC19-based backbone
Host strain: DH5α
Origin: Gibson assembly using pUC19 backbone from synthesized gBlocks and elements of the pOpt vectors from the Kruse Lab.
Insert: BleR-Clover fluorescent protein with C-terminal FLAG tag.
Selection: Ampicillin resistant
Comment: Transformed in to algae strain AES5.
Sproles AE, Berndt A, Fields FJ, Mayfield SP. Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins. Appl Microbiol Biotechnol. 2022 Feb;106(4):1677-1689. doi: 10.1007/s00253-022-11790-9. Epub 2022 Feb 7. PMID: 35129657; PMCID: PMC8882119.