pAphVIII (pPH075)

$30.00

From Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin October 2017

Streptomyces rimosus aminoglycoside-5 ́- phosphotransferase aphVIII selection marker optimized to C. reinhardtii codon bias, controlled by the HSP70A/RBCS2 tandem promoter and RBCS2 3’ UTR.

Selection in C. reinhardtii: paromomycin (10-40 μg/ml)
Host strain: DH10b
Selection in E. coli: ampicillin (100 µg/ml)
Link to sequence and map (GenBank, updated 09/2019)
Sequence (.docx file)

Overview of all CRISPR/Cas9 plasmids from the Hegemann lab

Visit www.chlamy.de for more info or contact CRISPR@chlamy.de


Greiner, A., Kelterborn, S., Evers, H., Kreimer, G., Sizova, I., and Hegemann, P. Targeting of photoreceptor genes via zinc-finger nucleases and CRISPR/Cas9 in Chlamydomonas reinhardtii. Plant Cell 30 (2017). https://doi.org/10.1105/tpc.17.00659