pAPN_crBSDr
$30.00
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Host strain: E. coli: DH5α
Selection in E. coli: Ampicillin
Vector: ColE1 derivate
Insert: BSD (Blasticidin S Deaminase, Blasticidin S resistance)
Base vector for easy construction of CRISPR donor plasmids with exchangeable selectable marker genes under the control of a RbcS2 promotor/intron/terminator. These vectors are digested with NheI and EcoRI and re-assembled by Gibson assembly with custom fragments up- and downstream of the resistance cassette to add homology arms, type IIs restriction sites and optionally fusion proteins.
For other resistances see pAB262-NAR-R, pAPN_AphVIII and pAPN_crAadA
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.