* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pAV2
$30.00
From Adrian Valli, David Baulcombe lab, University of Cambridge, UK, March 2016
This construct expresses a nitrate-inducible artificial miRNA against PSY. It confers resistance to ampicillin (carbenicillin) in E. coli, and resistance to paromomycin in transgenic Chlamydomonas lines. For further details, please see Valli et al., 2016, Genome Research. In this manuscript the plasmid is under the name of ni-amiRNA-PSY.
host strain: DH5α
amp resistant
Valli AA, Santos BA, Hnatova S, Bassett AR, Molnar A, Chung BY, Baulcombe DC (2016) Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs. Genome Res. 26:519-29