* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pAV4

Name: pAV4
SKU: P-908
Availability: InStock

$30.00

From Adrian Valli, David Baulcombe lab, University of Cambridge, UK, March 2016

This construct expresses the spectinomycin resistance gene (derived from pALM32) under a constitutive promoter. This construct in particular carries an intron in the middle of spectinomycin resistance gene that contains a miRNA stem-loop, which produces an artificial miRNA against Maa7. For further details, please see Valli et al., 2016, Genome Research. In this manuscript the plasmid is under the name of spect/intron(mi).

host strain: DH5α
amp resistant

Sequence

Valli AA, Santos BA, Hnatova S, Bassett AR, Molnar A, Chung BY, Baulcombe DC (2016) Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs. Genome Res. 26:519-29

pAV4

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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