* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pCEHC1-5.1H/S
$30.00
From Carolyn Silfow, University of Minnesota, February 2024
Vector: pBluescript KS (+)
Origin: A HindIII/SpeI fragment extending from Cre01: 1029777 – 1024634 in Phytozome v5.6 genomic DNA sequence was cloned into pBluescript KS (+) to create plasmid pCEHC1-5.1H/S.
Insert: Wild-type copy of Cre01.g005900 (CECH1 gene), nuclear DNA, 5144bp HindIII-SpeI fragment.
Comment: This plasmid can rescue the mutant phenotype of the cehc1 mutation in strain CC-4509.
Selection in E. coli: Ampicillin
Host strain: XL-1 Blue MRF’