pCM0-094

$30.00

From Pierre Crozet, Institute of Physico-Chemical Biology-Paris, December 2018

Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018).

Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B5
Brick name: 3Flag (with internal Lys, C-ter, CDS)
Brick type: Immuno- and purification tag
Brick function: C-terminal triple Flag tag with Chlamydomonas codon usage and a small sequence (AGAG) atthe beggining of the Flag tag, allowing a trypic finger printing of the protein (10 residues) even with a Lys at its end.
Source of Brick: Synthetized
Acceptor plasmid: pAGM1301
Designer / Builder: P. Crozet

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086