pCM0-TB_052
$30.00
From Alexander Einhaus and Dr. Thomas Baier, Bielefeld University-Germany, April 2022
Host strain: TOP10
Selection in E. coli: Streptomycin (50 μg/ml)
Position L0 (Patron et al): B3
Brick name: HlDXR (CDS_B3)
Brick type: Gene of interest (CDS)
Sequence reference: Uniprot: H2DQ70
Brick function: functional 1-deoxy-d-xylulose 5-phosphate reductoisomerase from Haematococcus lacustris, sequence optimized according to Baier et al. 2018
Source of Brick: Einhaus et al. 2022
Acceptor plasmid: pICH41258
Designer / Builder: A. Einhaus and T. Baier
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Baier T, Wichmann J, Kruse O, Lauersen KJ. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 2018 Jul 27;46(13):6909-6919. doi: 10.1093/nar/gky532. PMID: 30053227; PMCID: PMC6061784.
Einhaus A, Steube J, Freudenberg RA, Barczyk J, Baier T, Kruse O. Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production. Metab Eng. 2022 Jun 16;73:82-90. doi: 10.1016/j.ymben.2022.06.002. Epub ahead of print. PMID: 35717002.