pCM1-029
$30.00
From Félix de Carpentier, Institute of Physico-Chemical Biology-Paris, February 2020
Plasmid used in the Modular Cloning Toolkit (MoClo) from Crozet et al. (2018) and de Carpentier et al. (2020).
Host strain: NEB® 10-beta
Selection in E. coli: ampicillin (50 μg/ ml)
Position L1 (Weber et al): 2F
Brick name: PAR-BSR-TRBCS2 (TU)
Brick type: Antibiotic Resistance TU
Brick function: Resistance to Blasticidin in Chlamy
Source of Brick: Assembly of pCM0-020, pCM0-120 and pCM0-115
Acceptor plasmid: pICH47742
Designer / Builder: F. de Carpentier and J. Le Peillet
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086
de Carpentier et al. (2020) Blasticidin S deaminase: a new efficient selectable marker for Chlamydomonas reinhardtii. Frontiers in Plant Science 11:242