* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pIFT46-mNeonGreen

Name: pIFT46-mNeonGreen
SKU: P-1024 (amp)
Availability: InStock

$30.00

From Adrian Nievergelt, Pigino Group, Max-Planck Institute for Cell and Molecular Biology-Dresden, August 2022

Host strain: DH5α
Selection in E. coli: Ampicillin
Origin: pE345 (IFT46-YFP under a PSAD promotor)
Insert: genomic IFT46 with a replaced synthetic EcoRI-EcoRV fragment coding for mNeonGreen
Comment: Bright fluorescent green tag for IFT trains which is well suited for observation in TIRF or spinning disk microscopy. Confers resistance to paromomycin in Chlamydomonas.

Sequence

Nievergelt AP, Zykov I, Diener D, Chhatre A, Buchholz TO, Delling M, Diez S, Jug F, Štěpánek L, Pigino G. Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport. Curr Biol. 2022 Sep 26;32(18):4071-4078.e4. doi: 10.1016/j.cub.2022.07.033. Epub 2022 Aug 3. PMID: 35926510; PMCID: PMC9521741.

pIFT46-mNeonGreen

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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