pLM005

$30.00

From Luke Mackinder/Martin Jonikas, University of York, UK/Princeton, April 2017

This plasmid was designed for generating C-terminal CrYFP-3xFLAG fusion proteins. The plasmid can be cut with HpaI and then gene of interest inserted by Gibson assembly.

Selection: ampicillin in E. coli, paromomycin in Chlamydomonas

host strain: DH5α

Sequence details: https://www.ncbi.nlm.nih.gov/nuccore/KX077945.1

Sequence, FASTA & ApE files

Yang W, Wittkopp TM, Li X, Warakanont J, Dubini A, Catalanotti C, Kim RG, Nowack ECM, Mackinder LCM, Aksoy M, Dudley Page M, D’Adamo S, Saroussi S, Heinnickel M, Johnson X, Richaud P, Alric J, Boehm M, Jonikas MC, Benning C, Merchant S, Posewitz M, Grossman AR (2015) Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism. Proc Natl Acad Sci 112:14978-14983

Mackinder LCM, Meyer MT, Mettler-Altmann T, Chen V, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon concentrating organelle. Proc Natl Acad Sci 113:5958-5963