pLM006

$30.00

From Luke Mackinder/Martin Jonikas, University of York, UK/Princeton, April 2017

This plasmid was designed for generating C-terminal mCherry-3xHis fusion proteins. The plasmid can be cut with HpaI and then gene of interest inserted by Gibson assembly.

Selection: ampicillin in E. coli, hygromycin in Chlamydomonas

host strain: DH5α

Sequence details: https://www.ncbi.nlm.nih.gov/nuccore/KX077949.1

Sequence, FASTA & ApE files

Mackinder LCM, Meyer MT, Mettler-Altmann T, Chen V, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon concentrating organelle. Proc Natl Acad Sci 113:5958-5963