* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pLM155: pLM099-Cre09.g416850-Venus-3xFLAG
$30.00
From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020
RBMP2 (Cre09.g416850) coding sequence plus 2250 bp upstream of the start codon was cloned into pLM099 by recombineering to produce a C-terminal CrVenus-3xFLAG tag. Transformed into C. reinhardtii strain CMJ030 to produce CC-5647.
Host strain: DH10B
Selection in E. coli: kanamycin
Selection in C. reinhardtii: paromomycin
https://www.biorxiv.org/content/10.1101/2020.08.16.252858v1.full