* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pLM161
$30.00
From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020
Recombineering plasmid designed for generating C-terminal CrVenusYFP-3xFLAG fusion proteins.
Host strain: DB3.1 (streptomycin resistant, ccdB resistant)
Selection in E. coli: kanamycin
Selection in C. reinhardtii: hygromycin
Emrich-Mills TZ, Yates G, Barrett J, Girr P, Grouneva I, Lau CS, Walker CE, Kwok TK, Davey JW, Johnson MP, Mackinder LCM. A recombineering pipeline to clone large and complex genes in Chlamydomonas. Plant Cell. 2021 May 31;33(4):1161-1181. doi: 10.1093/plcell/koab024. PMID: 33723601; PMCID: PMC8633747.