pMBS298
$30.00
From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, July 2021
Host strain: TOP10
Selection in E. coli: spectinomycin (50 μg/ ml)
Position L0 (Patron et al): B2
Brick name: CTP PSAN (N-ter, CDS)
Brick type: Signal and Targeting peptides (CDS)
Brick function: Allows the targetting to the Chloroplast
Source of Brick: Cre02.g082500
Acceptor plasmid: pAGM1276
Designer / Builder: J. Niemeyer
The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/
Einhaus A, Baier T, Rosenstengel M, Freudenberg RA, Kruse O. Rational Promoter Engineering Enables Robust Terpene Production in Microalgae. ACS Synth Biol. 2021 Apr 16;10(4):847-856. doi: 10.1021/acssynbio.0c00632. Epub 2021 Mar 25. PMID: 33764741.