* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pMBS808

Name: pMBS808
SKU: M-134 (kan)
Availability: InStock

$30.00

From Justus Niemeyer, Michael Schroda lab, University of Kaiserslautern, March 2022

Host strain: TOP10
Selection in E. coli: kanamycin (50 μg/ ml)
Position L0 (Patron et al):
Brick name: L2-AphVIII-CRed (ParoR)
Brick type: Destination vector
Sequence reference:
Brick function: Destination vector with L1 aphVIII (ParoR) and CRed accepting L0 parts
Source of Brick: Niemeyer and Schroda 2022
Acceptor plasmid: pAGM4673
Designer / Builder: J. Niemeyer

The entire collection of MoClo Toolkit plasmids, distributed as frozen glycerol stocks in a 96-well format, can also be ordered for $400 for academic/non-profit users and $800 for commercial/industry here: https://www.chlamycollection.org/product/moclo-toolkit/

Sequence

Niemeyer J, Schroda M. New destination vectors facilitate Modular Cloning for Chlamydomonas. Curr Genet. 2022 Aug;68(3-4):531-536. doi: 10.1007/s00294-022-01239-x. Epub 2022 Apr 16. PMID: 35429260; PMCID: PMC9279246.

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Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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