pMJ013b

$30.00

From Martin Jonikas, Carnegie Institution for Science, Department of Plant Biology, Stanford, March 2014

This plasmid contains a PsaD promoter driving codon-optimized AphVIII, followed by a CYTc6 intron and PsaD 3’UTR. Digestion with MlyI yields a 2660bp fragment for transformation.

host strain: One Shot® TOP10 E. coli
amp resistant

 

Sequence


Zhang R, Patena W, Armbruster U, Gang SS, Blum SR, Jonikas MC (2014) High-throughput genotyping of green algal mutants reveals random distribution of mutagenic insertion sites and endonucleolytic cleavage of transforming DNA. Plant Cell 26:1398-1409