* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pMJ013b
$30.00
From Martin Jonikas, Carnegie Institution for Science, Department of Plant Biology, Stanford, March 2014
This plasmid contains a PsaD promoter driving codon-optimized AphVIII, followed by a CYTc6 intron and PsaD 3’UTR. Digestion with MlyI yields a 2660bp fragment for transformation.
host strain: One Shot® TOP10 E. coli
amp resistant
Zhang R, Patena W, Armbruster U, Gang SS, Blum SR, Jonikas MC (2014) High-throughput genotyping of green algal mutants reveals random distribution of mutagenic insertion sites and endonucleolytic cleavage of transforming DNA. Plant Cell 26:1398-1409