pMJ504: pLM005-Cre12.g498550-Venus-3xFLAG

$30.00

From Moritz Meyer, Martin Jonikas Lab, Princeton University, August 2020

MPM1 (Cre12.g498550) gDNA coding sequence, spanning the start codon to the triplet just before the stop codon, was cloned in frame into pLM005 by Gibson assembly (as described in https://doi.org/10.1073/pnas.1522866113. Transformed into C. reinhardtii strain cMJ030 to produce CC-5649.

Host strain: DH5α
Selection in E. coli: ampicillin
Selection in C. reinhardtii: paromomycin

Sequence