* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pMJ505: pLM005-Cre12.g498550-Venus-3xFLAG-3xCtermSAGA2
$30.00
From Moritz Meyer, Martin Jonikas Lab, Princeton University, August 2020
pMJ504, containing the gDNA of Cre12.g498550, was reengineered by restriction digestion and T4 ligation to add in-frame immediately after the 3xFLAG a synthetic fragment containing three copies of the sequence coding for the 15 C-terminal amino acids of SAGA2 (Cre 09.g394621), interspersed with a short flexible linker (GGGGGS). Transformed into C. reinhardtii strain cMJ030 to produce CC-5650.
Host strain: DH5α
Selection in E. coli: ampicillin
Selection in C. reinhardtii: paromomycin