pMJ505: pLM005-Cre12.g498550-Venus-3xFLAG-3xCtermSAGA2

$30.00

From Moritz Meyer, Martin Jonikas Lab, Princeton University, August 2020

pMJ504, containing the gDNA of Cre12.g498550, was reengineered by restriction digestion and T4 ligation to add in-frame immediately after the 3xFLAG a synthetic fragment containing three copies of the sequence coding for the 15 C-terminal amino acids of SAGA2 (Cre 09.g394621), interspersed with a short flexible linker (GGGGGS). Transformed into C. reinhardtii strain cMJ030 to produce CC-5650.

Host strain: DH5α
Selection in E. coli: ampicillin
Selection in C. reinhardtii: paromomycin

Sequence