* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pMJ508: pLM005-Cre10.g430350[W51A/R52A]-Venus-3xFLAG
$30.00
From Moritz Meyer, Martin Jonikas Lab, Princeton University, August 2020
Previously synthesized pMJ507 (CC-5652) was modified by side-directed mutagenesis. Triplet coding for Tryptophan in position 51 of the mature protein now codes for an Alanine. Triplet coding for Arginine in position 52 of the mature protein now codes for an Alanine. Transformed into C. reinhardtii strain cMJ030 to produce CC-5653.
Host strain: DH5α
Selection in E. coli: ampicillin
Selection in C. reinhardtii: paromomycin