* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pMJ509: pLM005-Cre10.g430350[W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A]-Venus-3xFLAG
$30.00
From Moritz Meyer, Martin Jonikas Lab, Princeton University, August 2020
Previously synthesized pMJ507 (CC-5652) was modified by side-directed mutagenesis. All four triplet pairs coding for a Tryptophan-Arginine dipeptide were mutagenized to code for a double Alanine (W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A). Transformed into C. reinhardtii strain cMJ030 to produce CC-5654.
Host strain: DH5α
Selection in E. coli: ampicillin
Selection in C. reinhardtii: paromomycin