* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pOpt2_PsaD_aadA_Min

Name: pOpt2_PsaD_aadA_Min
SKU: P-980
Availability: InStock

$30.00

From Kyle Lauersen, Olaf Kruse lab, Bielefeld University-Germany, May 2018

This is a minimized version of the pOpt2 vector which contains only a gene of interest cassette for reporter fusion to the aadA spectinomycin resistance gene. The vector has full complementarity to the pOpt2 vectors and contains the PsaD chloroplast targeting peptide between NdeI-BamHI. Fusion proteins can be generated for desired targets in fusion to the aadA and spectinomycin resistance allows direct selection for expressing transformants. This selection works whether fusion proteins are targeted to the algal chloroplast or the cytoplasm.

Host strain: DH5α
Selection in E. coli: ampicillin

Sequence

Wichmann J, Baier T, Wentnagel E, Lauersen KJ, Kruse O (2018) Tailored carbon partitioning for phototrophic production of (E)-α-bisabolene from the green microalga Chlamydomonas reinhardtii. Metab. Eng. 45:211–222

pOpt2_PsaD_aadA_Min

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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