* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pOpt_PβTUB2_mCerulean3_Paro

Name: pOpt_PβTUB2_mCerulean3_Paro
SKU: P-1112 (amp)
Availability: InStock

$30.00

From Severin Sasso, Leipzig University-Germany, April 2024

Vector: pOpt_mCerulean3_Paro with HSP70A-RBCS2 promoter (Lauersen et al., 2015)
Host Strain: TOP10
Origin: pOpt_mCerulean3_Paro digested with BcuI and NdeI; insert cloned upstream of the mCerulean3 coding sequence using primers with Gibson overhangs
Insert: β2-tubulin promoter; total insert length: 330 bp
Selection in E. coli: Ampicillin
Selection in C. reinhardtii: Paromomycin
Sequencing primer: 5’-TCCCATTCGCCATTCAGG-3’

Map

Sasso 2024 pOpt sequences

Lihanova Y, Nagel R, Jakob T, Sasso S. Characterization of activating cis-regulatory elements from the histone genes of Chlamydomonas reinhardtii. Plant J. 2024 May 1. doi: 10.1111/tpj.16781. Epub ahead of print. PMID: 38693717.

Scranton, M.A., Ostrand, J.T., Georgianna, D.R., Lofgren, S.M., Li, D., Ellis, R.C., et al. (2016) Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii. Algal Research, 15, 135–142.

Lauersen KJ, Kruse O, Mussgnug JH. Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Appl Microbiol Biotechnol. 2015 Apr;99(8):3491-503. doi: 10.1007/s00253-014-6354-7. Epub 2015 Jan 15. PMID: 25586579.

Brignon P, Chaubet N. Constitutive and cell-division-inducible protein-DNA interactions in two maize histone gene promoters. Plant J. 1993 Sep;4(3):445-57. doi: 10.1046/j.1365-313x.1993.04030445.x. PMID: 8220490.

pOpt_PβTUB2_mCerulean3_Paro

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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