* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pPF2-GcAMP6
$30.00
From Adrian Nievergelt, Pigino Group, Max-Planck Institute for Cell and Molecular Biology-Dresden, August 2022
Host strain: DH5α
Selection in E. coli: Ampicillin
Insert: DRC4 genomic with a c-terminal GcAMP6 reporter
Comment: A free calcium reporter which is fused to DRC4 (pf2)
Nievergelt AP, Zykov I, Diener D, Chhatre A, Buchholz TO, Delling M, Diez S, Jug F, Štěpánek L, Pigino G. Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport. Curr Biol. 2022 Sep 26;32(18):4071-4078.e4. doi: 10.1016/j.cub.2022.07.033. Epub 2022 Aug 3. PMID: 35926510; PMCID: PMC9521741.