pPF2-GcAMP6

$30.00

From Adrian Nievergelt, Pigino Group, Max-Planck Institute for Cell and Molecular Biology-Dresden, August 2022

Host strain: DH5α
Selection in E. coli: Ampicillin
Insert: DRC4 genomic with a c-terminal GcAMP6 reporter
Comment: A free calcium reporter which is fused to DRC4 (pf2)

Sequence


Nievergelt AP, Zykov I, Diener D, Chhatre A, Buchholz TO, Delling M, Diez S, Jug F, Štěpánek L, Pigino G. Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport. Curr Biol. 2022 Sep 26;32(18):4071-4078.e4. doi: 10.1016/j.cub.2022.07.033. Epub 2022 Aug 3. PMID: 35926510; PMCID: PMC9521741.