pRAM118
$30.00
From Sophie Skanchy, Martin Jonikas lab, June 2025
Created by: Silvia Ramundo
Plasmid: pRAM118 (empty backbone)
Origin: pRAM118 is derived from pLM005, but contains a hygromycin selectable marker in lieu of the paromomycin cassette. All other features remain the same.
E. coli host strain: Stellar competent cells (HST08)
Selection: ampicillin resistant in E. coli; hygromycin in Chlamydomonas
Itakura AK, Chan KX, Atkinson N, Pallesen L, Wang L, Reeves G, Patena W, Caspari O, Roth R, Goodenough U, McCormick AJ, Griffiths H, Jonikas MC. A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18445-18454. doi: 10.1073/pnas.1904587116. Epub 2019 Aug 27. PMID: 31455733; PMCID: PMC6744930.