* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pRed (a.k.a. pSC101-BAD-gbaA-tet)
$30.00
From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020
Recombineering plasmid containing the λ phage cassette under an L-arabinose inducible promoter and the temperature-sensitive SC101 origin of replication. Should be grown at 30°C, λ phage cassette expression activated at 37°C.
Host strain: DH5α
Selection in E. coli: tetracycline
pRed (a.k.a. pLM072) was a gift to Luke Mackinder from Mihail Sarov (Max Planck Institute, Dresden, Germany), see the following publication for details: https://www.nature.com/articles/nmeth933