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pRMC3

Name: pRMC3
SKU: P-1013
Availability: InStock

$30.00

From Anthony Berndt, Stephen Mayfield Lab, University of California, San Diego, November 2021

Source: Anthony Berndt, Stephen Mayfield Lab, University of California, San Diego
Plasmid: pRMC3
Vector: pUC19-based backbone
Host strain: DH5alpha
Origin: Gibson assembly using pUC19 backbone from synthesized gBlocks and elements of the pOpt vectors from the Kruse Lab.
Insert: ER-Golgi retained SARS-CoV-2 Spike RBD fused to mClover fluorescent protein.
Selection in E. coli: Ampicillin
Comment: Transformed in to algae strain RMC3. pRMC3 map in GenBank format also available in publication’s supplemental data.

Sequence

Berndt AJ, Smalley TN, Ren B, Simkovsky R, Badary A, Sproles AE, Fields FJ, Torres-Tiji Y, Heredia V, Mayfield SP. Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii. PLoS One. 2021 Nov 18;16(11):e0257089. doi: 10.1371/journal.pone.0257089. PMID: 34793485; PMCID: PMC8601568.

pRMC3

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Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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