* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pWEN2
$30.00
From Don Weeks, University of Nebraska, April 2017
pWEN2 is kanamycin resistant in E. coli and has the complete DNA sequence of the Cas9/intron-sgRNA gene targeting the native PDS3-1 gene.
host strain: DH5α
Sequence (ApE file)
Algal Research Cas9-intron-sgRNA Manuscript
Algal Research Supplemental Data
Wen Zhi Jiang, Donald P. Weeks, A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii, In Algal Research, Volume 26, 2017, Pages 474-480, ISSN 2211-9264
(http://www.sciencedirect.com/science/article/pii/S2211926417302138)