* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pWF.K.4

Name: pWF.K.4
SKU: M-186 (kan)
Availability: InStock

$30.00

From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024

Host strain: DH5α
Selection in E. coli: Kanamycin (50 µg/ml)
BsaI sites presence for homology arms cloning: No
BbsI sites for Level 2 cloning: Yes: TGCC and GGGA.
LacZα gene flanked by BbsI sites: Yes
Chloroplast 5′ and 3′ homology arms: Amplified from pWF vector (Kuras and Wollman, 1994)
Function: Level 2 Golden Gate compatible destination vector, containing Chlamydomonas plastome 5’ and 3’ homology regions (arms) to allow Chlamydomonas plastome transformation by homologous recombination in the pWF locus. For more information, refer to Melero-Cobo et al.

Sequence

Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.

pWF.K.4

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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