pWF.K.4
$30.00
From Pablo Leivar, IQS School of Engineering-Barcelona Spain, December 2024
Host strain: DH5α
Selection in E. coli: Kanamycin (50 µg/ml)
BsaI sites presence for homology arms cloning: No
BbsI sites for Level 2 cloning: Yes: TGCC and GGGA.
LacZα gene flanked by BbsI sites: Yes
Chloroplast 5′ and 3′ homology arms: Amplified from pWF vector (Kuras and Wollman, 1994)
Function: Level 2 Golden Gate compatible destination vector, containing Chlamydomonas plastome 5’ and 3’ homology regions (arms) to allow Chlamydomonas plastome transformation by homologous recombination in the pWF locus. For more information, refer to Melero-Cobo et al.
Melero-Cobo X, Gallemí M, Carnicer M, Monte E, Planas A, Leivar P. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering. Physiol Plant. 2025 Jan-Feb;177(1):e70088. doi: 10.1111/ppl.70088. PMID: 39871102; PMCID: PMC11772913.