Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre13.g562850-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: THF1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g495951-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CYN8
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre06.g264300-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PRPS15
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre10.g433950-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g520600-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PRPS6
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre03.g164300-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre06.g278195-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre02.g088850-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: RFS2
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre13.g562900-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre01.g016900-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre02.g118550-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre07.g325755-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre10.g423500-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: HMOX1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre03.g193950-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: FTR3
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre07.g312300-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: Heme oxygenase
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre13.g603500-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g493950-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PRPS13
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g411200-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: TEF5
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre02.g116250-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g651550-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: MTT1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g675550-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: FKB5
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g544114-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CYN20-6
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g405450-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre13.g580850-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PRPL22
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre07.g315150-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: RBD1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g556050-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PRPL9
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre10.g446350-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CGLD14
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre14.g615000-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g393765-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: LMR1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g490500-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CGL78
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