Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre05.g237350-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g671750-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PFD4
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre17.g723400-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre04.g219650-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre14.g617826-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: TIM22
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre03.g183550-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre01.g051500-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: LUP1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre13.g588700-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g393250-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g509050-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PSBP3
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre17.g741050-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: COV1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre17.g738000-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: AGG2
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre04.g225650-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre08.g373250-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: POA2
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g494250-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre06.g287100-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CAM11
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g535900-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: ARL1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre05.g244950-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: SSR2
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre08.g362900-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PSBP4
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre10.g418200-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g678350-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CAM19
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre06.g296600-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: RFS1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g415800-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PDCD4
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g387875-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: IPY3
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre05.g248150-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PGA2
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre10.g419800-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: PIS1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g527450-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: CAM17
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g689650-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: APG8
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g657550-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre17.g723650-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: ATO1
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