Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre07.g335100-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre17.g743747-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre01.g054500-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre13.g587200-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g548950-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: LHCBM7
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g687117-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre02.g087250-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre03.g170850-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: MMP19
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre03.g175850-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: EEP1
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre12.g516350-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: COX10
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre02.g102700-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g393210-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre03.g200431-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre01.g025200-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: MMP4
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre09.g389763-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre05.g242950-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: MRPL22
From Martin Jonikas, Princeton University, May 2022
cMJ030 expressing pLM005-Cre16.g693300-Venus-3xFLAG
The Chlamydomonas Spatial Interactome (CSI) collection was generated by Lianyong Wang/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_LW prefix).
Target genes, encoding chloroplast candidate proteins, were cloned into the pLM005 (Venus) vector and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications https://doi.org/10.1101/2022.05.31.493820
and at https://www.chlamylibrary.org/allTaggedStrains.
To minimize silencing we recommend propagating the CSI_LW strains on paromomycin.
Protein Name: –
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