Strains
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: Arg7, SpecR
Initial KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Second KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Third KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
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