Strains

From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025

Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Third KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component

Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.

From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025

Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Third KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component

Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.

From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025

Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Second KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Third KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component

Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.

From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025

Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Third KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase

Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.

From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025

Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: Arg7, SpecR
Initial KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Second KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Third KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component

Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.