Search Results for
Deposited by Simon Kelterborn and Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, February 2018
This is a chlamyopsin-1/2 (COP1/2) disruption strain, generated with CRISPR/Cas9.
Background strain CC-125
Nuclease (Sp)Cas9 as ribonucleoprotein (RNP)
Marker pAphVII (pPH360)
Target gene COP1/2, Cre01.g002500
Target sequence ATGGACTTCAAGAGCATCAGCGG (Exon 1)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of photoreceptor genes via zinc-finger nucleases and CRISPR/Cas9 in Chlamydomonas reinhardtii. Plant Cell 29, Issue 10
Deposited by Boris Zorin, Peter Hegemann lab, Humboldt University-Berlin, February 2018
This is a phototropin disruption strain, generated with single stranded DNA.
Background strain CC-4350
Nuclease no
Marker pAPHVIII
Target gene Phototropin, PHOT, Cre03.g199000
Overview of other strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This strain is no longer available. If other ∆phot strains are needed, please see the other phototropin (PHOT) disruption strains:
in CC-125: CC-5391 ∆PHOT-C41 [PH13], CC-5392 ∆PHOT-B5 [PH15]
in SAG11-32b: CC-5429 ∆PHOT-A5 [PH135], CC-5430 ∆PHOT-A7 [PH136]
Zorin B, Lu Y, Sizova I, Hegemann P (2009) Nuclear gene targeting in Chlamydomonas as exemplified by disruption of the PHOT gene. Gene 432(1-2):91-6
CC-5968 ∆SNRK2.2-C5 [PH158]
$30.00
$30.00
Deposited by Simon Kelterborn and Francisca Boehning, Peter Hegemann lab, Humboldt University of Berlin, December 2022
This is a SNRK2.2 (SAC3) disruption strain, generated with CRISPR/Cas9. Clone C5-A5.
Mutants with a disrupted SNRK2.2 (SAC3) gene show constitutive arylsulfatase expression and can phenotypically screened with X-SO4 dyes (see Kelterborn et al. 2022, doi.org/10.1007/978-1-0716-1791-5_3).
Background strain: SAG11-32b (=CC-409)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: SNRK2.2, Cre12.g499500
Target sequence: TAGCGAGGATGTCCAATCAG GGG (exon 1)
Mutation: insertion of short oligo (TTAGACTCTAACTAGATCAGcgg)
Overview of all CRISPR/Cas9 strains from the Hegemann lab: http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Simon Kelterborn and Francisca Boehning, Peter Hegemann lab, Humboldt University of Berlin, December 2022
This is a ChR1 disruption strain with a point mutation E123T in ChR2, generated with Zif and SpCas9 nucleases.
Background strain: CC-3403
Nuclease: SpCas9 and Zif nucleases
Target gene: ChR2, Cre02.g085257
Target sequence: ChR2 AGTGGTTGCGTTACGCCGAG (exon 6)
Marker: pAphVIII (pPH114), pArg+ (pPH230)
Mutation: E123T exchange in ChR2
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, December 2022.
This is a ChR1 disruption strain with a point mutation E90Q in ChR2, generated with SpCas9 nuclease in CC-125 and crossed into CC-124.
Background strain: CC-124 mt-
Nuclease: SpCas9 nuclease
Target gene: ChR1, Cre14.g611300; ChR2, Cre02.g085257
Target sequence: ChR1 TGTGGCTTCGTTACGCGGAG (exon 5);ChR2 CTATGTGTGCGCTATCGAGG (exon 4)
Mutation: E90R exchange in ChR2; insertion of short oligo TTTCGATTGAAGGAAAAGTTACAACGGAGT in ChR1
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, December 2022.
This is a ChR1 disruption strain with a point mutation E90Q in ChR2, generated with Zif and SpCas9 nucleases.
Background strain: CC-125 mt+
Nuclease: Zif and SpCas9 nucleases
Target gene: ChR1, Cre14.g611300; ChR2, Cre02.g085257
Target sequence: ChR1 TGTGGCTTCGTTACGCGGAG (exon 5); ChR2 CTATGTGTGCGCTATCGAGG (exon 4)
Marker: pAphVII (pPH360), pAphVIII (pPH114), pCrZ3 (pPH68)
Mutation: E90Q exchange in ChR2; insertion of short oligo TTTCGATTGAAGGAAAAGTTACAACGGAGT in ChR1
This strain was not published. Please contact irinasiz@yahoo.com before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, December 2022.
This is a ChR1 and ChR2 disruption strain, generated with SpCas9 nuclease.
Background strain: CC-125 mt+
Nuclease: SpCas9 nuclease
Target gene: ChR1, Cre14.g611300; ChR2, Cre02.g085257
Target sequence: ChR1 TGTGGCTTCGTTACGCGGAG (exon 5); ChR2 AGTGGTTGCGTTACGCCGAG (exon 6)
Marker: pAphVII (pPH360), pAphVIII (pPH114)
Mutation: insertion of short oligo TTTCGATTGAAGGAAAAGTTACAACGGAGT in ChR1 and ChR2
Baidukova O, Oppermann J, Kelterborn S, Fernandez Lahore RG, Schumacher D, Evers H, Kamrani YY, Hegemann P. Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation. Nat Commun. 2022 Nov 25;13(1):7253. doi: 10.1038/s41467-022-35018-6. PMID: 36433995; PMCID: PMC9700795.
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, December 2022.
This is a Rad51C disruption strain, generated with SpCas9 based on the CC-125 strain. Knock-out generated using CRISPR/Cas9 and FLAG sequence CGCCCACGCtAATTaGCGTGGGCGcgtga to disrupt the gene of interest. It contains FLAG sequence + an unidentified DNA sequence inserted at the disruption site.
Background strain: CC-125 mt+
Nuclease: SpCas9
Target gene: Rad51C, Cre02.g102500
Target sequence: TCTCGCCACTGGTTTCGGCA
Marker: pAphVII (pPH360)
This strain was not published. Please contact irinasiz@yahoo.com before using it.
CC-5952 ∆Ku80-G10 [PH096]
$30.00
$30.00
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, December 2022.
This is a KU80 disruption strain, generated with SpCas9 based on the CC-125 strain.
Background strain: CC-125 mt+
Nuclease: SpCas9
Knock-out generated using CRISPR/Cas9 and FLAG sequence ATAATGACCACGACATCGACTACAAGGACT to disrupt the gene of interest.
Target gene: KU80, Cre10.g423800
Target sequence: ggcggcaggaccagcctgga
Marker: pAphVII (pPH360)
This strain was not published. Please contact irinasiz@yahoo.com before using it.
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University-Berlin, April 2022
This is a pCRY (CPH1) disruption strain, generated with CRISPR/Cas9.
Background strain: ROC40-LUC+ (from Takuya Matsuo, see Niwa et al. 2013)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAphVII (pPH360)
Target gene: pCRY (CPH1), Cre06.g295200
Target sequence: GACCTAGAGTGTGATGCGCT (Exon7)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, September 2020
This is a aCry pCry disruption strain, generated with CRISPR/Cas9.
Background strain: CC-125 mt+
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAphVII (pPH360)
pAPHVIII (pPH75)
Target gene: pCry, Cre06.g295200
aCry, Cre06.g278251
Target sequence: GACCTAGAGTGTGATGCGCT
GAGCTCTGCTGGAGGAAATG
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, September 2020
This is a aCry pCry disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 73.72 mt+
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAphVII (pPH360)
pAPHVIII (pPH75)
Target gene: pCry, Cre06.g295200
aCry, Cre06.g278251
Target sequence: GACCTAGAGTGTGATGCGCT
GAGCTCTGCTGGAGGAAATG
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
CC-5640 ∆pCry-D7 [PH230]
$30.00
$30.00
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, September 2020
This is a pCry disruption strain, generated with CRISPR/Cas9.
Background strain: CC-125 mt+
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAphVII (pPH360)
Target gene: pCry, Cre06.g295200
Target sequence: GACCTAGAGTGTGATGCGCT
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
CC-5615 ∆COP1-F6 mt+ [PH239]
$30.00
$30.00
From Heide Evers, Hegemann lab, Humboldt University-Berlin, January 2020
Strain PH239 ∆COP1-F6 includes the 581 bp insert inside COP1
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University-Berlin, November 2019
This is a ChR1 ChR2 disruption strain, generated with CRISPR/Cas9 and Zinc-finger nuclease.
Background strain: CC-125 mt+
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Zinc-finger nuclease (ZFN)
Marker: pAPHVIII (pPH75)
pAphVII (pPH360)
Target gene: ChR1, Cre14.g611300
ChR2, Cre02.g085257
Target sequence: TGTGGCTTCGTTACGCGGAG TGG (ChR1, exon 5)
CTATGTGTGCGCTATCGAGG TGG (ChR2, exon 4)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
CC-5550 ∆ChR1-H6 mt+ [PH160]
$30.00
$30.00
Deposited by Simon Kelterborn and Francisca Boehning, Peter Hegemann lab, Humboldt University of Berlin, November 2019
This is a ChR1 disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 11-32b [=CC-409 mt+ = UTEX 90]
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: ChR1, Cre14.g611300
Target sequence: TGTGGCTTCGTTACGCGGAG TGG (exon 5)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Simon Kelterborn and Francisca Boehning, Peter Hegemann lab, Humboldt University of Berlin, November 2019
This is a ChR1 disruption strain, generated with CRISPR/Cas9.
Background strain: CC-3403 mt-
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pARG (pHR11)
Target gene: ChR1, Cre14.g611300
Target sequence: TGTGGCTTCGTTACGCGGAG TGG (exon 5)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Simon Kelterborn and Francisca Boehning, Peter Hegemann lab, Humboldt University of Berlin, November 2019
This is a CAV2 disruption strain, generated with CRISPR/Cas9.
Background strain: CC-5075 mt+ (sequence verified clone of CC-125)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: CAV2, Cre16.g665050
Target sequence: GGCGTACCGTCAATCAGCAT GGG (exon 5)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Simon Kelterborn and Francisca Boehning, Peter Hegemann lab, Humboldt University of Berlin, November 2019
This is a CiliK (GCLK1) disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 11-32b [=CC-409 mt+ = UTEX 90]
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: CiliK/GCLK1, Cre02.g104450
Target sequence: CCGGGATTCGAAGCGACGGA CGG (exon 1)
This strain was generated in a collaboration with Prof. William Snell.
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a PHOT/ UVR8 double disruption strain, generated with CRISPR/Cas9.
Background strain: CC-5392 ∆PHOT-B5 [PH15] (based on CC-125)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: UVR8, Cre05.g230600
Target sequence: CGGCGTCACAGTGACGAGTGTGG (Exon 6)
CC-5392 ∆PHOT-B5 [PH15] strain info:
Marker: pAphVII (pPH360)
Target gene: PHOT, Cre03.g199000
Target sequence: GCGCATCCTCAACTACACCAAGG (Exon 6)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a PHOT/ UVR8 double disruption strain, generated with CRISPR/Cas9.
Background strain: CC-5440 ∆UVR8-I8-E4 (based on CC-3403)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: PHOT, Cre03.g199000
Target sequence: GACTGGATATGGACCCGATGAGG (Exon 2)
CC-5440 ∆UVR8-I8-E4 mt- [PH050] strain info:
Marker: pArg
Target gene: UVR8, Cre05.g230600
Target sequence: CGAGGACAGATCTACAGCTGGGG (Exon2)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a COP5 disruption strain, generated with CRISPR/Cas9.
Background strain: CC-125 mt+
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAphVII (pPH360)
Target gene: COP5, Cre02.g074150
Target sequence: GCAGCACCTCCAGACTGACGCGG (Exon g6)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518
Deposited by Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a UVR8 disruption strain, generated with CRISPR/Cas9.
Background strain: CC-3403 (mt-)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pHR11
Target gene: UVR8, Cre05.g230600
Target sequence: CGAGGACAGATCTACAGCTGGGG (Exon 2)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518
CC-5436 ∆VGCC-H3 mt- [PH039]
$30.00
$30.00
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a VGCC disruption strain, generated with CRISPR/Cas9.
Background strain: CC-3403 (mt-)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pArg
Target gene: VGCC, g11458
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518
CC-5434 ∆VGCC-C9 mt+ [PH064]
$30.00
$30.00
Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a VGCC disruption strain, generated with CRISPR/Cas9.
Background strain: CC-125 mt+
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAphVII (pPH360)
Target gene: VGCC, g11458
Target sequence: GATCAAACCCCGCAAGTACGAGG (Exon2)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Sizova I, Greiner A, Awasthi M, Kateriya S, Hegemann P (2013) Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases. Plant J 73:873-82
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner from the Hegemann lab suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
From Andre Greiner, Hegemann lab, Humboldt University-Berlin, November 2015
Andre Greiner suggests that this strain be re-selected on Zeocin before analysis.
Trippens J, Greiner A, Schellwat J, Neukam M, Rottmann T, Lu Y, Kateriya S, Hegemann P, Kreimer G (2012) Phototropin influence on eyespot development and regulation of phototactic behavior in Chlamydomonas reinhardtii. Plant Cell 24:4687-702
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