B-Series CLiP Collection
The B-Series CLiP Collection (called the B-series for “Best”), consists of more than 13,000 mutants selected from the CLiP collection as having a confidence level of 1 or 2 and an insertion in either the CDS and/or intron region of genes. The mutants are supplied on 9 high-density (1536 colony/plate) agar plates. Users can screen plates individually for phenotypes of interest (for example acetate auxotrophy, high light sensitivity, etc.) if they have access to a replication robot. Alternatively, users can pool the mutants from one or all of the plates, subject them to a competitive growth assay in different media or different conditions, and then identify by sequencing those mutants that were selected under desired conditions. Conditions for pooling the library and conducting competitive growth experiments are described in Li et al., 2019 (cited below). Users can replicate the plates for their own research, but only for use in their own laboratories.
Plates will be available for a cost of $1000 for the set. This resource is not provided as a low-cost way of ordering multiple mutants, but rather for phenotypic screening. Therefore, the key to the identity of individual mutants on the plates will not be provided. As strains of interest are chosen using phenotypic screens or pooled growth experiments, the user can then order the individual strains from the CLiP collection for $100 per strain.
If your laboratory has used replica plating to identify strains of interest, the identity of any individual strain will be available upon request from the CRC, at a cost of $20 per strain. Send a picture of the plate with the clone(s) of interest highlighted to learn the identity of any of the strains.
When you receive the plates, please inspect them carefully. Remove any large condensation droplets from the lid that can occur during shipping. Keep them wrapped in parafilm and put them in a plastic box upon arrival to slow desiccation of the plate. We will replace any problem plates free-of-charge within 2 weeks of receipt. Send a photo of the damaged plate to Matt Laudon immediately. For best results, you should conduct your experiments using the library no more than 2 weeks after delivery.
Because of maintenance and replication factors, please be aware there will be a small number of empty positions on the arrayed plates. Plates will be shipped approximately 2 weeks after orders are received.
Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, Jonikas MC. A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18. PMID: 30886426; PMCID: PMC6636631.