CC-181 sr-u-2-23 mt+
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (streptomycin)
This is a subclone of the original sr-u-2-23 mutant, which was induced in the Boynton-Gillham lab at Duke, using nitrosoguanidine as mutagen.
This strain shows moderately low levels of streptomycin resistance (50-100 micrograms/ml) on minimal medium, and somewhat higher levels of resistance when grown on acetate. The mutation is a C>U change at position 856 in the chloroplast 16S rRNA, equivalent to position 912 of E. coli
Conde MF, Boynton JE, Gillham NW, Harris EH, Tingle CL, Wang WL (1975) Chloroplast genes in Chlamydomonas affecting organelle ribosomes. Genetic and biochemical analysis of antibiotic-resistant mutants at several gene loci. Mol Gen Genet 140:183-220
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292