CC-252 cr6 mt-

$30.00

Elizabeth Harris, Boynton-Gillham laboratory, Duke University

Phenotype: cold sensitive on minimal medium

The cr6 mutant is deficient in 70S chloroplast ribosomes and accumulates some 41S and some 54S subunit particles and/or poorly defined mixed subunit material. Initial characterization suggested that this mutant was distinguishable from another mutant, cr7, based on the amount of ribosomal subunit material accumulated when analyzed on sucrose gradients. In subsequent analysis by Myers et al. these two mutants proved to be indistinguishable, and were shown to be alleles at the same locus. Myers et al. showed that cr6 and cr7 fail to assemble to proteins of the large subunit of the chloroplast ribosome, one normally synthesized in the chloroplast and one in the cytoplasm.

The original cr6 mutant formed diploids with ac20, cr1, cr2, cr3, and cr4; cr5 was not tested.

Myers AM, Harris EH, Gillham NW, Boynton JE (1984) Mutations in a nuclear gene of Chlamydomonas cause the loss of two chloroplast ribosomal proteins, one synthesized in the chloroplast and the other in the cytoplasm. Curr Genet 8:369-378

Myers AM, Harris EH, Gillham NW, Boynton JE (1984) Mutations in a nuclear gene of Chlamydomonas cause the loss of two chloroplast ribosomal proteins, one synthesized in the chloroplast and the other in the cytoplasm. Curr Genet 8:369-378