* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pASapI
$30.00
From Saul Purton, University College London, August 2014
Chloroplast DNA sequence containing psbH-trnE2 region with an ‘expression cassette’ engineered into the intergenic MluI site. Cassette comprises of the atpA promoter/5’UTR and rbcL 3’UTR regions separated by a multiple cloning site. Note: the SapI site in the MCS is designed to allow a perfect translational fusion at the ATG of the gene-of-interest.
host strain: DH5α
amp resistant
Economou C, Wannathong T, Szaub J, Purton S (2014). A simple, low cost method for chloroplast transformation of the green alga Chlamydomonas reinhardtii. In: Chloroplast Biotechnology (volume editor: Pal Maliga). Methods in Molecular Biology 1132: 401-411