* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
Fea1_ble-2A-mCherry
$30.00
From Paula Barjona, University of Erlangen-Nuremberg, December 2018
The constitutive promoter HSP70A/Rbcs2 in plasmid pBR9 was replaced by the PCR-amplified FEA1 inducible promoter from plasmid p5’Fea1-ARS2 yielding Fea1_Ble2A-mCherry.
Host strain: DH5α
Selection in E. coli: ampicillin
Barjona do Nascimento Coutinho P, Friedl C, Buchholz R, Stute SC (2017) Chemical regulation of Fea1 driven transgene expression in Chlamydomonas reinhardtii. Algal Research. 26:323–329
Rasala BA, Barrera DJ, Ng J, Plucinak TM, Rosenberg JN, Weeks DP, Oyler GA, Peterson TC, Haerizadeh F, Mayfield SP (2013) Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. 74:545-56