* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5858 oda3 oda6::IC2-NG ift20::IFT20-mC mt-
$30.00
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by mating oda3 oda6::IC2-NG and ift20::IFT20-mCherry. The outer dynein arms (ODAs) are tagged on the essential IC2 subunits with mNeonGreen and IFT is visible by IFT20-mCheery expression. ODAs do not assemble in cilia due to the absence of the docking complex in oda3. This allows imaging of ODA transport by IFT.
Dai J, Barbieri F, Mitchell DR, Lechtreck KF. In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties. Mol Biol Cell. 2018 Oct 15;29(21):2553-2565. doi: 10.1091/mbc.E18-05-0291. Epub 2018 Aug 22. PMID: 30133350; PMCID: PMC6254574.