pOpt_4xEhist cons_PβTUB2_mCerulean3_Paro
$30.00
From Severin Sasso, Leipzig University-Germany, April 2024
Vector: pOpt_mCerulean3_Paro with HSP70A-RBCS2 promoter (Lauersen et al., 2015)
Host Strain: TOP10
Origin: pOpt_mCerulean3_Paro digested with BcuI and NdeI; inserts cloned upstream of the mCerulean3 coding sequence using primers with Gibson overhangs
Insert: Cis-regulatory module Ehist cons (Brignon and Chaubet, 1993) and β2-tubulin promoter; total length of two inserts: 521 bp
Selection in E. coli: Ampicillin
Selection in C. reinhardtii: Paromomycin
Sequencing primer: 5’-TCCCATTCGCCATTCAGG-3’
Lihanova Y, Nagel R, Jakob T, Sasso S. Characterization of activating cis-regulatory elements from the histone genes of Chlamydomonas reinhardtii. Plant J. 2024 May 1. doi: 10.1111/tpj.16781. Epub ahead of print. PMID: 38693717.
Scranton, M.A., Ostrand, J.T., Georgianna, D.R., Lofgren, S.M., Li, D., Ellis, R.C., et al. (2016) Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii. Algal Research, 15, 135–142.
Lauersen KJ, Kruse O, Mussgnug JH. Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Appl Microbiol Biotechnol. 2015 Apr;99(8):3491-503. doi: 10.1007/s00253-014-6354-7. Epub 2015 Jan 15. PMID: 25586579.
Brignon P, Chaubet N. Constitutive and cell-division-inducible protein-DNA interactions in two maize histone gene promoters. Plant J. 1993 Sep;4(3):445-57. doi: 10.1046/j.1365-313x.1993.04030445.x. PMID: 8220490.