CC-2062 fus1 mt+
$30.00
From Ursula Goodenough, Washington University, December 1986, originally from Matsuda
Phenotype: does not mate
The fus mutants are blocked in adhesion of mating structures prior to sexual fusion. This is a spontaneous allele at the FUS1 locus, isolated in Sager’s 21 gr strain by Matsuda and colleague
Also see CC-1158 for information on the imp1 allele at the same locus.
Matsuda Y, Tamaki S, Tsubo Y (1978) Mating type specific induction of cell wall lytic factor by agglutination of gametes in Chlamydomonas reinhardtii. Plant Cell Physiol 19:1253-1261
Matsuda Y, Tamaki S, Tsubo Y (1981) The isolation and properties of the lytic enzyme of the cell wall released by mating gametes of Chlamydomonas reinhardtii. Plant Cell Physiol 22:1607-1611
Saito T, Matsuda Y (1984) Sexual agglutinin of mating-type minus gametes in Chlamydomonas reinhardii. I. Loss and recovery of agglutinability of gametes treated with EDTA. Exp Cell Res 152:322-330
Forest CL (1987) Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J Cell Sci 88:613-621
Ferris PJ, Woessner JP, Goodenough UW (1996) A sex recognition glycoprotein is encoded by the plus mating-type gene fus1 of Chlamydomonas reinhardtii. Mol Biol Cell 7:1235-1248
Wang Q, Snell WJ (2003) Flagellar adhesion between mating type plus and mating type minus gametes activates a flagellar protein-tyrosine kinase during fertilization in Chlamydomonas. J Biol Chem 278:32936-32942