From Steve Pollock, Louisiana State University, March 3, 2003
To quickly and easily screen one or several strains of Chlamydomonas the following protocol works amazingly well:
1) resuspend a pellet of approximately 5-10 uL in size in 50 uL of 10mM NaEDTA by vortexing.
2) incubate at 100C for 5 minutes (thermocycler works well).
3) vortex to mix.
4) centifuge at 12000g for 1 minute.
5) use 1.5 uL in a 25 uL PCR reaction.