From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:
1) SPA1 (Cre03.g155051), chromosome_3 base 1902457 (genome v5.5), CDS – WARNING position has 73% probability of being correct
2) Cre03.g176833, chromosome_3 base 4558364 (genome v5.5), CDS – WARNING position has 58% probability of being correct
3) PGM12 (Cre06.g293150), chromosome_6 base 6509099 (genome v5.5), 3’UTR – position has 95% probability of being correct
4) PGM12 (Cre06.g293150), chromosome_6 base 6509112 (genome v5.5), 3’UTR – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.