BtetX
$30.00
From Sergio García and Guy Cardineau, Tecnológico de Monterrey, Campus Monterrey-Mexico, May 2015
The TetX open reading frame [Genbank: JQ990987] was synthesized de novo with codons optimized for Chlamydomonas reinhardtii cytoplasmic expression under the control of constitutive beta 2 tubulin promoter and chlamyopsin1 3’UTR.
Comment: The BtetX plasmid can be used to select nuclear transformants of Chlamydomonas reinhardtii. It has been proven to work in the cell wall deficient strain CC-849, however the wild-type cell-walled strain CC-125 is as sensitive to tetracycline as strain CC-849. The transformants should be selected in TAP plates containing 15 µg/mL of tetracycline and maintained below a light intensity of 24 µmoles m-2 s-1. Transformed colonies will be visible after 8 days and can resist up to 100 µg/mL of tetracycline.
host strain: DH5α
amp resistant