From Rachel Dent, Niyogi lab, University of California-Berkeley, 2011
The CAL collection of mutants affected in photosynthesis and/or photoprotection was generated in the Niyogi laboratory at UC-Berkeley by Rachel Dent et al. (Dent et al., 2005; Dent et al., 2015). This mutant was generated by insertional mutagenesis of the wild-type strain 4A+ (mt+, 137c background; CC-4051) using glass bead transformation with KpnI-linearized pMS188 plasmid (Schroda et al., 2002) conferring zeocin resistance. Flanking sequence adjacent to the resistance gene was isolated by a PCR-based approach.
Dent RM, Sharifi MN, Malnoë A, Haglund C, Calderon RH, Wakao S, Niyogi KK (2015) Large-scale insertional mutagenesis of Chlamydomonas supports phylogenomic functional prediction of photosynthetic genes and analysis of classical acetate-requiring mutants. Plant J. 82: 337-351
Dent RM, Haglund CM, Chin BL, Kobayashi MC, Niyogi KK (2005) Functional genomics of eukaryotic photosynthesis using insertional mutagenesis of Chlamydomonas reinhardtii. Plant Physiol. 137: 545-556
Schroda M, Beck CF, Vallon O (2002) Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas. Plant J. 31: 445–455
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