CC-125 wild type mt+ [137c]


Boynton-Gillham laboratory, Duke University

This is the basic “137c” wild type strain originally from G.M. Smith, isolated in 1945 near Amherst MA, and is presumably equivalent to strain 11/32c of the Culture Centre of Algae and Protozoa. This particular strain was brought to Duke by N.W. Gillham in 1968 from R.P. Levine’s laboratory at Harvard.

CC-124 and CC-125 carry the nit1 and nit2 mutations, and cannot grow on nitrate as their sole N source. CC-125 carries the AGG1 (agg1+) allele for phototactic aggregation, in contrast to CC-124, which has the agg1 allele at this locus.

CC-125 is the background strain for many mutations, including CC-503 cw92 mt+ which was the source of DNA used for genomic sequencing.

For more information on the origin of the standard laboratory strains of C. reinhardtii, please see the following paper:

Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610