CC-1860 pmp1 mt+
$30.00
From Martin Spalding, Iowa State University, October 1984
Phenotype: altered CO2 assimilation
Originally pmp-1-16-5k, this mutant was induced by 5-fluorodeoxyuridine plus ethyl methanesulfonate in the 2137 strain (see CC-3269). The pmp1 mutant is defective in inorganic carbon transport, and requires high CO2 concentrations for phototrophic growth. The pmp1 and ad (air-dier) mutants are alleles at the LCIB locus.
We maintain this strain in dim light.
Spalding MH, Spreitzer RJ, Ogren WL (1983) Reduced Inorganic Carbon Transport in a CO(2)-Requiring Mutant of Chlamydomonas reinhardii. Plant Physiol 73:273-276
Spalding MH, Spreitzer RJ, Ogren WL (1983) Genetic and physiological analysis of the CO2-concentrating system of Chlamydomonas reinhardii. Planta 159:261-266
Spalding MH, Ogren WL (1985) CO2 exchange characteristics during dark-light transitions in wild-type and mutant Chlamydomonas reinhardii cells. Photosynth Res 6:363-369
Moroney JV, Tolbert NE, Sears BB (1986) Complementation analysis of the inorganic carbon concentrating mechanism of Chlamydomonas reinhardtii. Mol Gen Genet 204:199-203
Wang Y, Spalding MH (2006) An inorganic carbon transport system responsible for acclimation specific to air levels of CO2 in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 103:10110-10115